Role of the amino-terminal region of the DnaA protein in opening of the duplex DNA at the oriC region

被引：0
|
作者：
Mima, S
Yamagachi, Y
Kondo, T
Tsuchiya, T
Mizushima, T
机构：
[1] Okayama Univ, Fac Pharmaceut Sci, Okayama 7008530, Japan
[2] Japan Sci & Technol Corp, PRESTO, Kawaguchi 3320012, Japan
来源：
FEMS MICROBIOLOGY LETTERS | 1999年 / 176卷 / 01期
关键词：
DNA replication; DnaA; N-terminal region; site-directed mutagenesis;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We report in this paper that the amino acid residues Ile-26 and Leu-40 of the DnaA protein are essential for the DNA replication activity in vitro. Lines of evidence to support this conclusion are as follows. Variants of the DnaA protein containing either an Ile-26-Ser or Leu-40-Ser replacement were unable to support oriC DNA replication in vitro. Though the mutant DnaA proteins retained the capability to bind oriC DNA, they were unable to open the duplex DNA at oriC. Based on these and other results, we conclude that the N-terminal region of the DnaA protein is involved in the oligomerization of this protein, an essential step for the duplex opening activity at oriC, (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：163 / 167
页数：5
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