Comparison of Commercial and In-House Real-Time PCR Assays Used for Detection of Mycoplasma pneumoniae

被引：21

作者：

Dumke, Roger ^{[1
]}

Jacobs, Enno ^{[1
]}

机构：

[1] Tech Univ Dresden, Med Fac Carl Gustav Carus, Inst Med Microbiol & Hyg, D-01307 Dresden, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 02期

关键词：

P1 CYTADHESIN GENE; CHLAMYDOPHILA-PNEUMONIAE; LEGIONELLA-PNEUMOPHILA; CHLAMYDIA-PNEUMONIAE; RESPIRATORY SAMPLES; CLINICAL STRAINS; INFECTIONS; SEQUENCE; MANIFESTATIONS; EPIDEMIOLOGY;

D O I：

10.1128/JCM.01989-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We tested two commercial and three in-house PCR assays under standardized conditions to detect Mycoplasma pneumoniae. All five procedures were able to demonstrate M. pneumoniae DNA in a concentration comparable to 1 CFU/mu l, but the mean crossing points resulted in differences in the concentration of the genome copies of a factor of 20.

引用

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页码：441 / 444

页数：4

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