Mesenchymal Stem Cell Secreted-Extracellular Vesicles are Involved in Chondrocyte Production and Reduce Adipogenesis during Stem Cell Differentiation

被引:11
|
作者
Tsai, Yu-Chen [1 ]
Cheng, Tai-Shan [2 ]
Liao, Hsiu-Jung [3 ]
Chuang, Ming-Hsi [4 ,5 ]
Chen, Hui-Ting [6 ,7 ,13 ]
Chen, Chun-Hung [8 ]
Zhang, Kai-Ling [9 ]
Chang, Chih-Hung [10 ,11 ]
Lin, Po-Cheng [5 ]
Huang, Chi-Ying F. [1 ,2 ,12 ]
机构
[1] Natl Yang Ming Chiao Tung Univ, Dept Biotechnol & Lab Sci Med, Taipei 11221, Taiwan
[2] Natl Yang Ming Chiao Tung Univ, Inst Biopharmaceut Sci, Taipei 11221, Taiwan
[3] Far Eastern Mem Hosp, Dept Med Res, New Taipei 220216, Taiwan
[4] Chung Hua Univ, Coll Management, Hsinchu 30012, Taiwan
[5] Gwo Xi Stem Cell Appl Technol Co Ltd, Hsinchu 30261, Taiwan
[6] Natl Yang Ming Chiao Tung Univ, Dept Pharm, Taipei 11221, Taiwan
[7] Kaohsiung Med Univ, Dept Fragrance & Cosmet Sci, Kaohsiung 80708, Taiwan
[8] GTESTing Lab, Hsinchu 30261, Taiwan
[9] Natl Yang Ming Chiao Tung Univ, Coll Biol Sci & Technol, Hsinchu 30010, Taiwan
[10] Far Eastern Mem Hosp, Dept Orthoped, New Taipei 22060, Taiwan
[11] Yuan Ze Univ, Grad Sch Biotechnol & Bioengn, Taoyuan 32003, Taiwan
[12] Kaohsiung Med Univ, Dept Biochem, Kaohsiung 80708, Taiwan
[13] Kaohsiung Med Univ, Sch Pharm, Kaohsiung 80708, Taiwan
关键词
Cartilage regeneration; Ultrafiltration; Extracellular vesicles; Mesenchymal stem cells; KNEE OSTEOARTHRITIS; ADIPOCYTE DIFFERENTIATION; ALCIAN BLUE; EXOSOMES; MICRORNAS; INJECTION; CARTILAGE; DISEASE; OBESITY; MIR-27A;
D O I
10.1007/s13770-022-00490-0
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Background: Extracellular vesicles (EVs) are derived from internal cellular compartments, and have potential as a diagnostic and therapeutic tool in degenerative disease associated with aging. Mesenchymal stem cells (MSCs) have become a promising tool for functional EVs production. This study investigated the efficacy of EVs and its effect on differentiation capacity. Methods: The characteristics of MSCs were evaluated by flow cytometry and stem cell differentiation analysis, and a production mode of functional EVs was scaled from MSCs. The concentration and size of EVs were quantitated by Nanoparticle Tracking Analysis (NTA). Western blot analysis was used to assess the protein expression of exosome-specific markers. The effects of MSC-derived EVs were assessed by chondrogenic and adipogenic differentiation analyses and histological observation. Results: The range of the particle size of adipose-derived stem cells (ADSCs)- and Wharton's jelly -MSCs-derived EVs were from 130 to 150 nm as measured by NTA, which showed positive expression of exosomal markers. The chondrogenic induction ability was weakened in the absence of EVs in vitro. Interestingly, after EV administration, type II collagen, a major component in the cartilage extracellular matrix, was upregulated compared to the EV-free condition. Moreover, EVs decreased the lipid accumulation rate during adipogenic induction. Conclusion: The results indicated that the production model could facilitate production of effective EVs and further demonstrated the role of MSC-derived EVs in cell differentiation. MSC-derived EVs could be successfully used in cell-free therapy to guide chondrogenic differentiation of ADSC for future clinical applications in cartilage regeneration.
引用
收藏
页码:1295 / 1310
页数:16
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