Development of the Fc-III Tagged Protein Expression System for Protein Purification and Detection

被引：3
|
作者：
Feng, Shan [1 ]
Tian, Enbing [2 ]
Zhang, Lei [1 ]
Wang, Qingtao [2 ]
Deng, Haiteng [1 ]
机构：
[1] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
[2] Capital Med Univ, Beijing Chaoyang Hosp, Beijing, Peoples R China
来源：
PLOS ONE | 2012年 / 7卷 / 08期
基金：
美国国家卫生研究院; 中国国家自然科学基金;
关键词：
RECOMBINANT PROTEINS; FUSION PROTEINS; BINDING;
D O I：
10.1371/journal.pone.0044208
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In the present work, we developed the Fc-III tagged protein expression system for protein purification and detection. The Fc-III sequence encodes for a 13 residue peptide and this peptide is cyclized by disulfide bond formation when the fusion protein is expressed. The Fc-III-fusion proteins selectively bind to immunoglobulin Fc domains (IgG-Fc) expressed from E. coli. We showed the efficient purification of Fc-III tagged proteins by immobilized non-native IgG-Fc and the detection of the cellular locations of fusion proteins by fluorescent-conjugated IgG-Fc. Our results prove that Fc-III tagged protein expression system is a simple and efficient tool for protein purification and detection and is a useful addition to the biochemistry and proteomics toolbox.
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收藏
页数：6
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