A TIME-RESOLVED FLUORESCENCE IMMUNOSENSING PLATFORM FOR HIGHLY SENSITIVE DETECTION OF CARDIAC TROPONIN I

被引:0
|
作者
Kim, K. R. [1 ]
Chun, H. J. [1 ]
Han, Y. D. [1 ]
Lee, K. W. [1 ]
Hong, D. K. [2 ]
Lee, K. N. [2 ]
Yoon, H. C. [1 ]
机构
[1] Ajou Univ, Suwon, South Korea
[2] Korea Elect Technol Inst, Seongnam, South Korea
关键词
Time-resolved fluorescence analysis; silica nanoparticle; TRF immunoassay; lanthanide; cardiac troponin I; IMMUNOASSAYS;
D O I
暂无
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
In this study, we developed a novel time-resolved fluorescence (TRF)-based immunosensing platform that consisted with the physically stable europium chelate-encapsulated silica nanoparticle and the simplified TRF analyzer. To verify the applicability of developed TRF immunosensing platform toward practical immunoassay, the sandwich immunosensing of cardiac troponin I (cTnI) was accomplished using the synthesized europium-encapsulated silica nanoparticles as a signaling probe and homemade TRF analysis system as an optical transducer.
引用
收藏
页码:1640 / 1643
页数:4
相关论文
共 50 条
  • [21] Establishment and Application of a Dual-Labeling Time-Resolved Fluorescence Immunoassay Method for Simultaneous Detection of the Troponin I-C Complex and Full-Size-Troponin I
    Huang, Biao
    Wu, Jian
    Chen, Hao
    Zhang, Li
    Zhou, Xiumei
    Wu, Qingqing
    Li, Ting
    Wang, Yigang
    Xia, Penguo
    Dai, Yaping
    Kai, Guoyin
    Liu, Pengfei
    Pei, Hao
    FRONTIERS IN CARDIOVASCULAR MEDICINE, 2021, 7
  • [22] Highly sensitive chromatographic time-resolved fluoroimmunoassay for rapid onsite detection of streptomycin in milk
    Wang, Zile
    Sun, Yuanze
    Liang, Demei
    Zeng, Yuyang
    He, Shuang
    Mari, Ghulam Mujtaba
    Peng, Tao
    Jiang, Haiyang
    JOURNAL OF DAIRY SCIENCE, 2020, 103 (10) : 8750 - 8760
  • [23] Highly sensitive time-resolved fluoroimmunoassay for the quantitative onsite detection of Alternaria longipes in tobacco
    Ji, Yuan
    Hu, Liwei
    Xiong, Wei
    Wang, Ying
    Yang, Fei
    Shi, Mowen
    Zhang, Haiyan
    Shao, Jimin
    Lu, Canhua
    Fang, Dunhuang
    Deng, Huimin
    Bian, Zhaoyang
    Tang, Gangling
    Liu, Shili
    Fan, Ziyan
    Liu, Shanshan
    JOURNAL OF APPLIED MICROBIOLOGY, 2022, 132 (02) : 1250 - 1259
  • [24] Spectra-resolved technique of a sensitive time-resolved fluorescence immunoassay instrument
    Guo, ZY
    Tian, Z
    Jia, YL
    ALT'03 INTERNATIONAL CONFERENCE ON ADVANCED LASER TECHNOLOGIES: BIOMEDICAL OPTICS, 2003, 5486 : 222 - 226
  • [25] Time-resolved fluorescence detection of mosaic DNA chip
    Zhuzhou Institute of Technology, Zhuzhou 412008, China
    不详
    不详
    不详
    不详
    J. Nanosci. Nanotechnol., 2006, 1 (66-71):
  • [26] Time-resolved fluorescence detection of mosaic DNA chip
    He, QG
    Chen, H
    Nie, LB
    Tang, JX
    Xiao, PF
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2006, 6 (01) : 66 - 71
  • [27] A homogeneous time-resolved fluorescence detection of telomerase activity
    Gabourdes, M
    Bourgine, V
    Mathis, G
    Bazin, H
    Alpha-Bazin, W
    ANALYTICAL BIOCHEMISTRY, 2004, 333 (01) : 105 - 113
  • [28] Active CMOS biochip for time-resolved fluorescence detection
    Patounakis, G
    Shepard, KL
    Levicky, R
    2005 SYMPOSIUM ON VLSI CIRCUITS, DIGEST OF TECHNICAL PAPERS, 2005, : 68 - 71
  • [29] TIME-RESOLVED FLUORESCENCE DETECTION OF HCV PCR PRODUCTS
    HAYASHI, PH
    ZELDIS, JB
    VANDENBRINK, KM
    SHERMAN, DG
    GODSEY, JH
    HEPATOLOGY, 1992, 16 (04) : A205 - A205
  • [30] An automated PCR platform with homogeneous time-resolved fluorescence detection and dry chemistry assay kits
    Hagren, Virve
    von Lode, Plia
    Syrjala, Anniina
    Soukka, Tero
    Lovgren, Timo
    Kojola, Hannu
    Nurmi, Jussi
    ANALYTICAL BIOCHEMISTRY, 2008, 374 (02) : 411 - 416