A TIME-RESOLVED FLUORESCENCE IMMUNOSENSING PLATFORM FOR HIGHLY SENSITIVE DETECTION OF CARDIAC TROPONIN I

被引:0
|
作者
Kim, K. R. [1 ]
Chun, H. J. [1 ]
Han, Y. D. [1 ]
Lee, K. W. [1 ]
Hong, D. K. [2 ]
Lee, K. N. [2 ]
Yoon, H. C. [1 ]
机构
[1] Ajou Univ, Suwon, South Korea
[2] Korea Elect Technol Inst, Seongnam, South Korea
关键词
Time-resolved fluorescence analysis; silica nanoparticle; TRF immunoassay; lanthanide; cardiac troponin I; IMMUNOASSAYS;
D O I
暂无
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
In this study, we developed a novel time-resolved fluorescence (TRF)-based immunosensing platform that consisted with the physically stable europium chelate-encapsulated silica nanoparticle and the simplified TRF analyzer. To verify the applicability of developed TRF immunosensing platform toward practical immunoassay, the sandwich immunosensing of cardiac troponin I (cTnI) was accomplished using the synthesized europium-encapsulated silica nanoparticles as a signaling probe and homemade TRF analysis system as an optical transducer.
引用
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页码:1640 / 1643
页数:4
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