Novel gold nanobiosensor platforms for rapid and inexpensive detection of Vibrio cholerae

Vibrio cholerae is a Gram-negative curved-rod bacterium belonging to the Vibrionaceae family. Routine detection of V. cholerae infection can be achieved by isolation of the organism from stool sample on the selective medium, followed by biochemical tests and specific antibodies for serotyping and serogrouping. These methods are labor-working and time-consuming. Furthermore, they provide low sensitivity and specificity. Advanced diagnostic approaches for identification of V. cholerae, such as cell-counting techniques by immunoassay, PCR, and real-time PCR are also used. In addition to these strategies, nanotechnology including gold (Au) or silver nanoparticles and carbon nanotubes (CNTs) hold great promise for rapid, accurate, and cost-effective detection of pathogens. In recent years, composites based on golden-graphene nanoparticles have been synthesized using electrochemical methods. They are capable of detecting very low copy numbers of DNA from Vibrio spp. owing to the synergistic effect between the graphene and gold nanoparticles. Therefore, development of nanobiosensors using the gold nanoparticles combined the golden-graphene binary platform nanobiosensor that will open new avenues for the efficient V. cholerae identification. Copyright (C) 2020 Wolters Kluwer Health, Inc. All rights reserved.