Analysis of the BACE1 and Clusterin Genes Expression Levels in Alzheimer's Disease

被引：2

作者：

Alsaadoni, Hani ^{[1
]}

Caykara, Burcu ^{[1
]}

Pence, Sadrettin ^{[1
]}

Tuzgen, Saffet ^{[2
]}

Pence, Halime Hanim ^{[3
]}

机构：

[1] Istanbul Univ, Aziz Sancar Inst Expt Med ASDETAE, Dept Mol Med, Istanbul, Turkey

[2] Istanbul Univ Cerrahpasa, Cerrahpasa Sch Med, Dept Neurosurg, Istanbul, Turkey

[3] Univ Hlth Sci, Sch Med, Dept Med Biochem, Istanbul, Turkey

来源：

JOURNAL OF ACADEMIC RESEARCH IN MEDICINE-JAREM | 2019年 / 9卷 / 01期

关键词：

BACE1; clusterin; alzheimer's disease; gene expression; PROTEIN;

D O I：

10.5152/jarem.2019.2671

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: This study aimed to explore the mRNA expressions of the beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) and clusterin (CLU) genes in blood samples of patients with Alzheimer's disease (AD) and healthy subjects. Methods: The expression levels of CLU and BACE1 in blood samples of subjects were examined using the real-time polymerase chain reaction (RT-PCR). The expression levels of patient and control groups were calculated using the 2-Delta Delta Ct method. The Statistical Package for Social Sciences (SPSS) version 22 (IBM Corp., Armonk, NY, USA) was used for statistical analysis, and a p of <0.05 was accepted as statistically significant. Results: The BACE1 and CLU genes were found 14-fold and 13-fold more expressed in the blood of the AD group than on the control group, respectively. Conclusion: Our study showed that the blood mRNA levels of the BACE1 and CLU genes were associated with AD.

引用

页码：45 / 49

页数：5

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