Analysis of the BACE1 and Clusterin Genes Expression Levels in Alzheimer's Disease

被引：2

作者：

Alsaadoni, Hani ^{[1
]}

Caykara, Burcu ^{[1
]}

Pence, Sadrettin ^{[1
]}

Tuzgen, Saffet ^{[2
]}

Pence, Halime Hanim ^{[3
]}

机构：

[1] Istanbul Univ, Aziz Sancar Inst Expt Med ASDETAE, Dept Mol Med, Istanbul, Turkey

[2] Istanbul Univ Cerrahpasa, Cerrahpasa Sch Med, Dept Neurosurg, Istanbul, Turkey

[3] Univ Hlth Sci, Sch Med, Dept Med Biochem, Istanbul, Turkey

来源：

JOURNAL OF ACADEMIC RESEARCH IN MEDICINE-JAREM | 2019年 / 9卷 / 01期

关键词：

BACE1; clusterin; alzheimer's disease; gene expression; PROTEIN;

D O I：

10.5152/jarem.2019.2671

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: This study aimed to explore the mRNA expressions of the beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) and clusterin (CLU) genes in blood samples of patients with Alzheimer's disease (AD) and healthy subjects. Methods: The expression levels of CLU and BACE1 in blood samples of subjects were examined using the real-time polymerase chain reaction (RT-PCR). The expression levels of patient and control groups were calculated using the 2-Delta Delta Ct method. The Statistical Package for Social Sciences (SPSS) version 22 (IBM Corp., Armonk, NY, USA) was used for statistical analysis, and a p of <0.05 was accepted as statistically significant. Results: The BACE1 and CLU genes were found 14-fold and 13-fold more expressed in the blood of the AD group than on the control group, respectively. Conclusion: Our study showed that the blood mRNA levels of the BACE1 and CLU genes were associated with AD.

引用

页码：45 / 49

页数：5

共 50 条

[1] BACE1 in Alzheimer's disease
Sathya, M.
Premkumar, P.
Karthick, C.
Moorthi, P.
Jayachandran, K. S.
Anusuyadevi, M.
[J]. CLINICA CHIMICA ACTA, 2012, 414 : 171 - 178
[2] BACE1 expression and activity: Relevance in Alzheimer's disease
Zacchetti, Daniele
Chieregatti, Evelina
Bettegazzi, Barbara
Mihailovich, Marija
Sousa, Vitor Lino
Grohovaz, Fabio
Meldolesi, Jacopo
[J]. NEURODEGENERATIVE DISEASES, 2007, 4 (2-3) : 117 - 126
[3] Platelet BACE1 levels as a possible biomarker for Alzheimer’s disease
Boris Decourt
Aaron Walker
Amanda Gonzales
Michael Malek-Ahmadi
Carolyn Liesback
Kathryn Davis
Christine Belden
Sandra Jacobson
Marwan Sabbagh
[J]. Molecular Neurodegeneration, 8 (Suppl 1)
[4] The β-Secretase BACE1 in Alzheimer's Disease
Hampel, Harald
Vassar, Robert
De Strooper, Bart
Hardy, John
Willem, Michael
Singh, Neeraj
Zhou, John
Yan, Riqiang
Vanmechelen, Eugeen
De Vos, Ann
Nistico, Robert
Corbo, Massimo
Imbimbo, Bruno Pietro
Streffer, Johannes
Voytyuk, Iryna
Timmers, Maarten
Monfared, Amir Abbas Tahami
Irizarry, Michael
Albala, Bruce
Koyama, Akihiko
Watanabe, Naoto
Kimura, Teiji
Yarenis, Lisa
Lista, Simone
Kramer, Lynn
Vergallo, Andrea
[J]. BIOLOGICAL PSYCHIATRY, 2021, 89 (08) : 745 - 756
[5] BACE1 gene variants do not influence BACE1 activity, levels of APP or Aβ isoforms in CSF in Alzheimer's disease
Sjolander, Annica
Zetterberg, Henrik
Andreasson, Ulf
Minthon, Lennart
Blennow, Kaj
[J]. MOLECULAR NEURODEGENERATION, 2010, 5
[6] Advancements in BACE1 and non-peptide BACE1 inhibitors for Alzheimer's disease
Shah, Nishita P.
Solanki, Vivek S.
Gurjar, Archana S.
[J]. INDIAN JOURNAL OF CHEMISTRY SECTION B-ORGANIC CHEMISTRY INCLUDING MEDICINAL CHEMISTRY, 2018, 57 (06): : 830 - 842
[7] The Alzheimer's disease β-secretase enzyme, BACE1
Cole, Sarah L.
Vassar, Robert
[J]. MOLECULAR NEURODEGENERATION, 2007, 2 (1)
[8] The Alzheimer's disease β-secretase enzyme, BACE1
Sarah L Cole
Robert Vassar
[J]. Molecular Neurodegeneration, 2
[9] Development of BACE1 inhibitors for Alzheimer's disease
Guo, Tao
Hobbs, Doug W.
[J]. CURRENT MEDICINAL CHEMISTRY, 2006, 13 (15) : 1811 - 1829
[10] BACE1 -: The β-secretase enzyme in Alzheimer's disease
Vassar, R
[J]. JOURNAL OF MOLECULAR NEUROSCIENCE, 2004, 23 (1-2) : 105 - 113

← 1 2 3 4 5 →