Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

被引：28
|
作者：
Boutaoui, Nassima [1 ]
Zaiter, Lahcene [1 ]
Benayache, Fadila [1 ]
Benayache, Samir [1 ]
Carradori, Simone [2 ]
Cesa, Stefania [3 ]
Giusti, Anna Maria [4 ]
Campestre, Cristina [2 ]
Menghini, Luigi [2 ]
Innosa, Denise [5 ]
Locatelli, Marcello [2 ]
机构：
[1] Univ Freres Mentouri, Unite Rech Valorisat Ressources Nat, Mol Bioact & Analyses Physicochim & Biol, Constantine 1,Route Ain El Bey, Constantine 25000, Algeria
[2] Univ G dAnnunzio, Dept Pharm, Via Vestini 31, I-66100 Chieti, Italy
[3] Sapienza Univ Roma, Dipartimento Chim & Tecnol Farmaco, Ple Aldo Moro 5, I-00185 Rome, Italy
[4] Sapienza Univ Roma, Dipartimento Med Sperimentale, Ple Aldo Moro 5, I-00185 Rome, Italy
[5] Univ Teramo, Fac Biosci & Tecnol Agroalimentari & Ambientali, Via Renato Balzarini 1, I-64100 Teramo, Italy
来源：
MOLECULES | 2018年 / 23卷 / 02期
关键词：
color analysis; pigments; MAE; HPLC-PDA; SFE; Thymus algeriensis; CHEMICAL-COMPOSITION; ESSENTIAL OILS; ANTIMICROBIAL ACTIVITY; BIOLOGICAL-ACTIVITIES; CHLOROPHYLL-A; PLANTS; ANTIOXIDANT; PRODUCTS;
D O I：
10.3390/molecules23020463
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This study was performed to evaluate the metabolite recovery from different extraction methods applied to Thymus algeriensis aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min at 100 degrees C gave the most phenolics-enriched extract, reducing extraction time without degradation effects on bioactives. Sixteen compounds were identified in this extract, 11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis and determination of chlorophylls and carotenoids implemented the knowledge of the chemical profile of this plant.
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页数：11
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