Rotational resonance NMR of 13C2-labelled retinal:: quantitative internuclear distance determination

Rotational resonance phenomena are investigated in the solid-state magic-angle spinning NMR of all-E-[11,20-C-13(2)]-retinal at a magnetic field of 4.7 T. We find good agreement between experiments and numerical simulations for the rotational resonance spectral peakshapes and for the rotor-driven magnetization exchange. The internuclear distance between the C-13-labelled C11 and C20 sites is determined to be 0.301 +/- 0.008 nm (from rotational resonance spectra) and 0.300 +/- 0.010 nm (from rotor-driven magnetization exchange), in agreement with the X-ray crystallographic distance of 0.296 nm. We show rotational resonance spectra which display perturbations from intermolecular homonuclear spin-spin interactions. (C) 1999 Elsevier Science B.V. All rights reserved.