Application of simple biomechanical and biochemical tests to heart valve leaflets: Implications for heart valve characterization and tissue engineering

被引:25
|
作者
Huang, Hsiao-Ying S. [1 ]
Balhouse, Brittany N. [2 ]
Huang, Siyao [1 ]
机构
[1] N Carolina State Univ, Dept Mech & Aerosp Engn, Raleigh, NC 27695 USA
[2] N Carolina State Univ, Dept Biomed Engn, Raleigh, NC 27695 USA
关键词
Heart valves; real-time maps; mechanical property; collagen; microstructure; BIAXIAL MECHANICAL-PROPERTIES; SMOOTH-MUSCLE-CELLS; AORTIC-VALVE; BIOLOGICAL-MATERIALS; PULMONARY VALVE; REPLACEMENT; BEHAVIOR; CUSP;
D O I
10.1177/0954411912455004
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A simple biomechanical test with real-time displacement and strain mapping is reported, which provides displacement vectors and principal strain directions during the mechanical characterization of heart valve tissues. The maps reported in the current study allow us to quickly identify the approximate strain imposed on a location in the samples. The biomechanical results show that the aortic valves exhibit stronger anisotropic mechanical behavior than that of the pulmonary valves before 18% strain equibiaxial stretching. In contrast, the pulmonary valves exhibit stronger anisotropic mechanical behavior than aortic valves beyond 28% strain equibiaxial stretching. Simple biochemical tests are also conducted. Collagens are extracted at different time points (24, 48, 72, and 120 h) at different locations in the samples. The results show that extraction time plays an important role in determining collagen concentration, in which a minimum of 72 h of extraction is required to obtain saturated collagen concentration. This work provides an easy approach for quantifying biomechanical and biochemical properties of semilunar heart valve tissues, and potentially facilitates the development of tissue engineered heart valves.
引用
收藏
页码:868 / 876
页数:9
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