A convenient and rapid method for genetic transformation of E-coli with plasmids

被引：14
|
作者：
Chen, XD [1 ]
Guo, PY [1 ]
Xie, ZX [1 ]
Shen, P [1 ]
机构：
[1] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
来源：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2001年 / 80卷 / 3-4期
关键词：
Escherichia coli; plasmid; genetic transformation;
D O I：
10.1023/A:1013040812987
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A convenient and rapid method for the genetic transformation of Escherichia coli with plasmids is proposed. By mixing the recipient cells and plasmid DNA and spreading them directly on selective medium plates containing Ca2+, the so-called `plate transformation' could achieve almost the same transformation efficiency as the classical transformation method with calcium. The whole protocol takes only about 2 min, its simplicity compared favorably, not only to the usual protocol, but also to all other documented modifications.
引用
收藏
页码：297 / 300
页数：4
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