Rapid typing of truffle mycorrhizal roots by PCR amplification of the ribosomal DNA spacers

被引：39
|
作者：
Mello, A
Nosenzo, C
Meotto, F
Bonfante, P
机构：
[1] CNR,CTR STUDIO MICOL TETTENO,I-10125 TURIN,ITALY
[2] UNIV TURIN,DIPARTIMENTO BIOL VEGETALE,I-10125 TURIN,ITALY
来源：
MYCORRHIZA | 1996年 / 6卷 / 05期
关键词：
polymerase chain reaction; ectomycorrhiza; Tuber; ribosomal genes;
D O I：
10.1007/s005720050141
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
DNA analyses were developed to type mycorrhizas of two Tuber species of commercial value (T. melanosporum, T. borchii) and a competitive fungus (Sphaerosporella brunnea) which forms ectomycorrhizas with plants usually considered hosts for truffles. Polymerase chain reaction (PCR) amplification of DNA isolated from fruitbodies, mycelia, mycorrhizas and leaves of host plants, was performed with a primer pair for an internal transcribed spacer ITS1-4. ITS amplification followed by restriction fragment length polymorphism (RFLP) analysis of the amplified products clearly distinguished the two Tuber species at the fruitbody, mycorrhiza and mycelium levels.
引用
收藏
页码：417 / 421
页数：5
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