Direct protein identification from nonspecific peptide pools by high-accuracy MS data filtering

被引：13
|
作者：
Thieu, Vinh An
Kirsch, Dieter
Flad, Thomas
Mueller, Claudia
Spengler, Bernhard [1 ]
机构：
[1] Univ Giessen, Inst Anorgan & Analyt Chem, D-35392 Giessen, Germany
[2] Univ Tubingen, Med Klin & Poliklin, Abt 2, Sekt Transplantat Immunol & Immunhamatol, D-72072 Tubingen, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 20期
关键词：
high mass accuracy; mass spectrometry; peptide sequencing; protein structures; sequence determination;
D O I：
10.1002/anie.200503787
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Validate your results! High-accuracy mass spectrometric analysis and composition-based de novo sequencing (CBS) combined with database(DB)-assisted protein identification allows for the rapid production of validated analytical results in proteome research. A simplified sample preparation scheme is sufficient to provide for reliable identification of proteins even when following nonspecific and unknown degradation processes. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：3317 / 3319
页数：3
相关论文
共 50 条
  • [21] Activity science with data from the upcoming generation of space-based high-accuracy photometric data
    Favata, F
    Aigrain, S
    ASTRONOMISCHE NACHRICHTEN, 2002, 323 (3-4) : 283 - 287
  • [22] An automated method for identification and quantification of protein and peptide markers in mammalian samples using LC-MS/MS data
    Pettersen, Harald
    Fenyo, David
    Lindqvist, Staffan
    Bonilla, Leo
    Richmond-Chew, Tori
    Bjorkesten, Lennart
    MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (10) : S259 - S259
  • [23] Towards high-accuracy bacterial taxonomy identification using phenotypic single-cell Raman spectroscopy data
    Li, Guangyu
    Wang, Zijian
    Wu, Chieh
    Wang, Dongqi
    Han, Il
    Lee, Jangho
    Kaeli, David R.
    Dy, Jennifer G.
    Weinberger, Kilian Q.
    Gu, April Z.
    ISME COMMUNICATIONS, 2025, 5 (01):
  • [24] High-Accuracy Facial Depth Models derived from 3D Synthetic Data
    Khan, Faisal
    Basak, Shubhajit
    Javidnia, Hossein
    Schukat, Michael
    Corcoran, Peter
    2020 31ST IRISH SIGNALS AND SYSTEMS CONFERENCE (ISSC), 2020, : 279 - 283
  • [25] Museum genomics: low-cost and high-accuracy genetic data from historical specimens
    Rowe, Kevin C.
    Singhal, Sonal
    Macmanes, Matthew D.
    Ayroles, Julien F.
    Morelli, Toni Lyn
    Rubidge, Emily M.
    Bi, Ke
    Moritz, Craig C.
    MOLECULAR ECOLOGY RESOURCES, 2011, 11 (06) : 1082 - 1092
  • [26] Protein and peptide identification algorithms using MS for use in high-throughput, automated pipelines
    Shadforth, I
    Crowther, D
    Bessant, C
    PROTEOMICS, 2005, 5 (16) : 4082 - 4095
  • [27] Identification of the components of simple protein mixtures by high accuracy peptide mass mapping and database searching
    Jensen, ON
    Podtelejnikov, AV
    Mann, M
    ANALYTICAL CHEMISTRY, 1997, 69 (23) : 4741 - 4750
  • [28] High-accuracy identification of interferograms between two vortex beams via deep learning without adequate experimental data
    Rui-Jia, Lu
    Zhi-Kun, Su
    JOURNAL OF OPTICS, 2023, 25 (03)
  • [29] AlphaFamlmpute: high-accuracy imputation in full-sib families from genotype-by-sequencing data
    Whalen, Andrew
    Gorjanc, Gregor
    Hickey, John M.
    BIOINFORMATICS, 2020, 36 (15) : 4369 - 4371
  • [30] A high-accuracy, time-saving method for extracting nest watch data from video recordings
    Evans, Dean R.
    McArthur, Sarah L.
    Bailey, Jacob M.
    Church, John S.
    Reudink, Matthew W.
    JOURNAL OF ORNITHOLOGY, 2015, 156 (04): : 1125 - 1129
12345