Label-free electrostatic detection of DNA amplification by PCR using capacitive field-effect devices

被引：3
|
作者：
Poghossian, A. [1 ,2 ]
Bronder, T. S. [1 ,2 ]
Scheja, S. [1 ]
Wu, C. [1 ,5 ]
Weinand, T. [4 ]
Metzger-Boddien, C. [4 ]
Keusgen, M. [3 ]
Schoening, M. J. [1 ,2 ]
机构：
[1] FH Aachen, Inst Nano & Biotechnol, Campus Julich, D-52428 Julich, Germany
[2] Forschungszentrum Julich GmbH, Peter Grunberg Inst PGI 8, D-52425 Julich, Germany
[3] Univ Marburg, Inst Pharmaceut Chem, D-35032 Marburg, Germany
[4] Gerbion GmbH & Co KG, D-70806 Kornwestheim, Germany
[5] Xi An Jiao Tong Univ, Sch Med, Inst Med Engn, Xian, Peoples R China
来源：
PROCEEDINGS OF THE 30TH ANNIVERSARY EUROSENSORS CONFERENCE - EUROSENSORS 2016 | 2016年 / 168卷
关键词：
field; effect sensor; DNA hybridization; polymerase chain reaction (PCR); label-free detection; polyelectrolyte; ADDRESSABLE POTENTIOMETRIC SENSOR; POLYELECTROLYTE LAYER; NUCLEIC-ACIDS;
D O I：
10.1016/j.proeng.2016.11.512
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process. (C) 2016 The Authors. Published by Elsevier Ltd.
引用
收藏
页码：514 / 517
页数：4
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