Seed protein profiles and cultivar identification in garden pea (Pisum sativum L.)

Electrophoretic patterns of seed proteins of 23 genotypes of garden pea (Pisum sativum L.) when analyzed by SDS-PAGE were placed in 22 groups on the basis of banding pattern. All the genotypes showed different banding patterns except PMR-43 and Azad Pea-3 which fell in the same group and showed similar banding pattern. The similarity between two genotypes might be possible due to their derivation from the cross of same parental combination. On the basis of distance matrix and UPGMA analysis, dissimilarity between genotypes was established and most dissimilar genotypes viz., Bonneville vs PMR-43, Bonneville vs Azad Pea-3, Bonneville vs VL-7 and NDVP-9 vs IP-3 (Pant Uphar) were identified. Seed protein electrophoresis is thus useful in identifying pea cultivars and in breeding programme.