Effects of ozone on sugar beet grown in open-top chambers

Sugar beet (Beta vulgaris cv. Patriot) plants were grown on field plots and in open-top chambers (OTCs) in two successive years. In the OTC treatments, plants were exposed to charcoal filtered air, unfiltered air or unfiltered air enriched with additional ozone (O-3). Ozone exposure continued for almost 5 months and the 8-h average concentration was raised from 34 to 39 nL L-1 in the ambient air chambers to 62 nL L-1 in the ozone enriched chambers. In both years, the AOT40 exposure index in the ozone enriched chambers exceeded 30 mu L L-1 h during the 5-month exposure period compared to 6.5 and 2.9 mu L L-1 h in ambient air in 2003 and 2004, respectively. Visible symptoms in the form of small white necrotic flecks appeared in both seasons in the ozone enriched chambers. When the data for both years were analysed statistically, a significant reduction of root yield of 6% and a slight reduction of sugar content were detected. These changes resulted in an overall reduced sugar yield ha(-1) of about 9%. Although the sensitivity of sugar beet to ozone is highly variety-dependent, in general this biennial crop appears less sensitive than annual crops such as wheat and potato. Ozone has limited effects on quality parameters in sugar beet, although an increase in alpha-amino-N content was observed, in agreement with the increased nitrogen content resulting from ozone exposure of wheat and potato. Enclosure within the OTCs increased aboveground biomass but decreased root yield (fresh biomass) and sugar content. These effects were most likely caused by a reduction of radiation by the chamber walls and annulus. The increased temperature in the chambers reduced yield quality by increasing mineral content. (c) 2006 Elsevier B.V. All rights reserved.