High-Throughput Structural Elucidation of Amides in Mallotus lianus Croiz Plant Materials by LC-ESI-MS-MS

Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) was utilized to elucidate the tentative structures of amides in Mallotus lianus Croiz plant materials. Collision-induced dissociation was performed in order to propose the structural information for ten unknown amides on the basis of the fragmentation pathways of the four purified amides, which were supported by their corresponding high resolution FTICR-MS data. This is the first report about the structural identification of amides in M. lianus Croiz by LC-ESI-MS-MS directly, which could eliminate the disadvantages such as tedious and material consuming of traditional phytochemical analysis.