CHARACTERIZATION OF TRYPSIN-LIKE ENZYMES FROM THE MIDGUT OF MORIMUS FUNEREUS (COLEOPTERA: CERAMBYCIDAE) LARVAE

被引：11

作者：

Loncar, N. ^{[1
]}

Bozic, Natasa ^{[2
]}

Nenadovic, Vera ^{[3
]}

Ivanovic, Jelisaveta ^{[3
]}

Vujcic, Z. ^{[1
]}

机构：

[1] Univ Belgrade, Fac Chem, Dept Biochem, Belgrade 11000, Serbia

[2] Univ Belgrade, Inst Chem Technol & Met, Ctr Chem, Belgrade 11000, Serbia

[3] Sinisa Stankovic Inst Biol Res, Dept Insect Physiol & Biochem, Belgrade 11060, Serbia

来源：

ARCHIVES OF BIOLOGICAL SCIENCES | 2009年 / 61卷 / 04期

关键词：

Cerambycidae; Morimus funereus; trypsin; midgut peptidases; synthetic substrate; zymogram; TENEBRIO-MOLITOR COLEOPTERA; DIGESTIVE PROTEINASES; PARTIAL-PURIFICATION; NEUROSECRETION;

D O I：

10.2298/ABS0904713L

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine.

引用

页码：713 / 718

页数：6

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