Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods

被引：7
|
作者：
Reis, Luciana Pimenta [1 ]
Miranda Menezes, Liliane Denize [2 ]
Lima, Graciela Kunrath [1 ]
de Souza Santos, Ethiene Luiza [1 ]
Seles Dorneles, Elaine Maria [3 ]
Sampaio de Assis, Debora Cristina [1 ]
Lage, Andrey Pereira [1 ]
Cancado, Silvana de Vasconcelos [1 ]
de Figueiredo, Tadeu Chaves [1 ]
机构：
[1] Univ Fed Minas Gerais, Escola Vet, BR-31270901 Belo Horizonte, MG, Brazil
[2] IMA, Contagem, MG, Brazil
[3] Univ Fed Lavras UFLA, Dept Vet Med, Lavras, MG, Brazil
来源：
CIENCIA RURAL | 2018年 / 48卷 / 02期
关键词：
C; jejuni; coli; detection methods; VIDAS (R) 30; CHICKEN CARCASSES; JEJUNI; DIFFERENTIATION; IDENTIFICATION; ASSAYS; MEAT; COLI; DNA;
D O I：
10.1590/0103-8478cr20161034
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS (R) 30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS (R) 30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni.
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页数：7
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