Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods

被引：7

作者：

Reis, Luciana Pimenta ^{[1
]}

Miranda Menezes, Liliane Denize ^{[2
]}

Lima, Graciela Kunrath ^{[1
]}

de Souza Santos, Ethiene Luiza ^{[1
]}

Seles Dorneles, Elaine Maria ^{[3
]}

Sampaio de Assis, Debora Cristina ^{[1
]}

Lage, Andrey Pereira ^{[1
]}

Cancado, Silvana de Vasconcelos ^{[1
]}

de Figueiredo, Tadeu Chaves ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Escola Vet, BR-31270901 Belo Horizonte, MG, Brazil

[2] IMA, Contagem, MG, Brazil

[3] Univ Fed Lavras UFLA, Dept Vet Med, Lavras, MG, Brazil

来源：

CIENCIA RURAL | 2018年 / 48卷 / 02期

关键词：

C; jejuni; coli; detection methods; VIDAS (R) 30; CHICKEN CARCASSES; JEJUNI; DIFFERENTIATION; IDENTIFICATION; ASSAYS; MEAT; COLI; DNA;

D O I：

10.1590/0103-8478cr20161034

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS (R) 30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS (R) 30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni.

引用

页数：7

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