Crystallization and preliminary crystallographic study of HIP/PAP, a human C-lectin overexpressed in primary liver cancers

被引：16
|
作者：
Abergel, C
Chenivesse, S
Stinnakre, MG
Guasco, S
Bréchot, C
Claverie, JM
Devinoy, E
Christa, L
机构：
[1] Inst Biol Struct & Microbiol, CNRS, UMR 1889, F-13402 Marseille 20, France
[2] CHU Necker, INSERM, U370, F-75742 Paris 15, France
[3] CHU Necker, Liver Unit, F-75742 Paris 15, France
[4] INRA, Unite Biol Cellulaire & Mol, F-78352 Jouy En Josas, France
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444999007969
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Human HIP/PAP is an adhesion protein expressed in normal pancreatic and Paneth cells and overexpressed in hepatocellular carcinoma. HIP/PAP was crystallized using the Hampton Research Crystal Screen and SAmBA software to define the optimal crystallization protocol. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), With unit-cell parameters a = 30.73, b = 49.35, c = 92.15 Angstrom and one molecule in the asymmetric unit. Flash-frozen crystals diffract to 1.78 Angstrom resolution using synchrotron. radiation. A molecular-replacement solution was obtained using the human Reg/ lithostathine structure and the AMoRe software.
引用
收藏
页码：1487 / 1489
页数：3
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