Crystallization and preliminary crystallographic study of HIP/PAP, a human C-lectin overexpressed in primary liver cancers

被引：16

作者：

Abergel, C

Chenivesse, S

Stinnakre, MG

Guasco, S

Bréchot, C

Claverie, JM

Devinoy, E

Christa, L

机构：

[1] Inst Biol Struct & Microbiol, CNRS, UMR 1889, F-13402 Marseille 20, France

[2] CHU Necker, INSERM, U370, F-75742 Paris 15, France

[3] CHU Necker, Liver Unit, F-75742 Paris 15, France

[4] INRA, Unite Biol Cellulaire & Mol, F-78352 Jouy En Josas, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444999007969

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human HIP/PAP is an adhesion protein expressed in normal pancreatic and Paneth cells and overexpressed in hepatocellular carcinoma. HIP/PAP was crystallized using the Hampton Research Crystal Screen and SAmBA software to define the optimal crystallization protocol. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), With unit-cell parameters a = 30.73, b = 49.35, c = 92.15 Angstrom and one molecule in the asymmetric unit. Flash-frozen crystals diffract to 1.78 Angstrom resolution using synchrotron. radiation. A molecular-replacement solution was obtained using the human Reg/ lithostathine structure and the AMoRe software.

引用

页码：1487 / 1489

页数：3

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