Regulation of cytosolic phospholipase A2 expression by cytokines in human amnion cells

The metabolism of arachidonic acid results in the production of prostaglandins (PGs), which are involved in the initiation of labour at term and preterm. The fetal membranes are a source of pro-inflammatory cytokines which promote increased PG biosynthesis via increased release of arachidonic acid and its conversion to biologically active metabolites such as PGE(2) and PGF(2 alpha) In the amnion, the liberation of arachidonic acid from membrane glycerophospholipid stores can be catalysed by cytosolic phospholipase A(2) (cPLA(2)). In amnion-derived WISH cells, the addition of tumour-necrosis factor alpha (TNF-alpha) (50 ng/ml) provoked a time-dependent increase in the expression of the cPLA(2) mRNA which aias greatest at 8 and 16 h post-treatment (3.62 +/- 0.52 and 3.15 +/- 0.45-fold of control, n = 3). The increase in cPLA(2) mRNA expression by TNF-alpha was unaffected by the prior addition of interleukin-4 (IL-4) (10 ng/ml), a known inhibitor of prostaglandin endoperoxide H synthase (PGHS)-2 mRNA and protein expression in WISH cells. TNF-alpha also increased the level of immunoreactive cPLA(2) protein in a time-dependent manner with the highest levels evident after 8 and 16 h. As with the mRNA, cPLA(2) protein levels were unaffected by pre-incubation with IL-4. The inclusion of the cPLA(2)-specific inhibitor arachidonyl trifluoromethyl ketone (AACOCF(3)) resulted in a concentration-dependent inhibition of PGE(2) biosynthesis in WISH cells treated with TNF-alpha (>95 per cent at 2 mu M). We conclude that TNF-alpha increases the abundance of the cPLA(2) mRNA and protein in amnion epithelial cells, an effect which plays an important role in amnion PG biosynthesis in the presence of intrauterine infection. (C) 1999 W. B. Saunders Company Ltd.