Evaluation of genetic diversity of Chinese Pleurotus ostreatus cultivars using DNA sequencing technology

被引：4
|
作者：
Liu, Yu [1 ]
Wang, Shouxian [1 ]
Yin, Yonggang [1 ]
Xu, Feng [1 ]
机构：
[1] Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
来源：
ANNALS OF MICROBIOLOGY | 2013年 / 63卷 / 02期
关键词：
EF1; alpha; Internal transcribed spacer; Phylogenetic analyses; Pleurotus ostreatus; RNA polymerase IIu; MUSHROOMS; BASIDIOMYCOTA; AGARICALES; STRATEGIES; ERYNGII; RPB2;
D O I：
10.1007/s13213-012-0505-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Pleurotus spp. are well-known and economically important cultivated mushrooms in China. Knowledge of the genetic relationship between the Chinese cultivars is essential to the improvement of P. ostreatus strains. Sequence analysis of the internal transcribed spacers (ITS), translation elongation factor (EF1 alpha) and the second largest subunit of RNA polymerase II (RPB2) was performed to assess the genetic diversity of Pleurotus ostreatus strains cultivated in China. The phylogenetic tree constructed using the combined results of the ITS, EF1 alpha and RPB2 sequence analyses showed the genetic relationships between the studied strains. Our phylogenetic analyses therefore provided valuable information on the relationships among the P. ostreatus strains used in this study and that was useful for examining genetic diversity among these strains.
引用
收藏
页码：571 / 576
页数：6
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