Production of extracellular lipases by Bacillus sp GCB-3 in relation to the nutritional conditions

The present study deals with the microbial biosynthesis of lipases by a strain of Bacillus spp. GCB-3. Fifteen strains were isolated from oily products These strains were screened for the production of lipases by solid state fermentation in 250ml Erlenmeyer flasks Of all the strains examined, GCB-3 gave maximum production of lipases (33 9 +/- 2 U/g). Different agricultural by-products such, as wheat bran, rice husk, almond meal, cotton seed meal, soybean meal, sunflower meal and mustard meal were used as substrates Maximum extracellular lipase activity (33.9 +/- 2 U/g) was observed when almond meal was used as the substrate 0.5% of Tween 80 was optimized for the maximum production of lipase (42 58 0.8 U/g).