Characterization of Native Bacillus Thuringiensis (Berliner) Isolates from India

被引：1
|
作者：
Anandhi, P. [1 ]
Saravanan, L. [2 ]
Ramtake, P. W. [3 ]
Elamathi, S. [4 ]
Simon, Sobita [1 ]
Varma, Savita [5 ]
机构：
[1] Deemed Univ, Allahabad Agr Inst, Dept Plant Protect, Allahabad 211007, Uttar Pradesh, India
[2] Directorate Oil Palm, Pedavegi, AP, India
[3] Deemed Univ, Dept Biol Sci, Allahabad Agr Inst, Allahabad 211007, Uttar Pradesh, India
[4] Deemed Univ, Allahabad Agr Inst, Dept Agron, Allahabad 211007, Uttar Pradesh, India
[5] Mahatma Gandhi Chitrakooot Gramodaya Vishwavidyal, Satna, MP, India
来源：
NATIONAL ACADEMY SCIENCE LETTERS-INDIA | 2012年 / 35卷 / 04期
关键词：
Bacillus thuringiensis; Chemical characterization; Electron microscopy; Pericalia ricini; PROTEINS;
D O I：
10.1007/s40009-012-0017-y
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Five native isolates of Bacillus thuringiensis (B.t) were morphologically and biologically characterized. Gram staining, capsule staining, endospore staining and transmission electron microscopy confirmed the B.t. by morphologically. Poly-acrylamide-gel-electrophoresis showed that all the native isolates were producing 130-140 kDa proteins. Chemical characterization revealed that Isolates NSC-1, NSC-3 and COR-4 were fermented maltose while NSC-9 and GUR-5 showed delayed +ve reaction. All selected cultures were catalase +ve, ammonification -ve, citrate utilization +ve, casein hydrolysis +ve, gelatin hydrolysis -ve, indole production -ve and VP test -ve. In the bioassays, five native strains NSC-1, NSC-3, NSC-9, COR-4 and GUR-5 were identified and proved as a novel isolate for controlling Castor hairy caterpillar (Pericalia ricini, Arctidae; Lepidoptera).
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页码：243 / 247
页数：5
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