Production of 10(S)-hydroxy-8(E)-octadecenoic and 7,10(S,S)-hydroxy-8(E)-octadecenoic ethyl esters by Novozym 435 in solvent-free media

被引：1
|
作者：
Martin-Arjol, Ignacio [3 ]
Busquets, Montse [1 ]
Isbell, Terry A. [2 ]
Manresa, Angels [3 ]
机构：
[1] Univ Barcelona, Fac Biol, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain
[2] ARS, Natl Ctr Agr Utililizat Res, USDA, Peoria, IL USA
[3] Univ Barcelona, Fac Farm, Dept Microbiol & Parasitol Sanitaries, E-08028 Barcelona, Spain
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2013年 / 97卷 / 18期
关键词：
Lipase; Novozym; 435; trans-Hydroxy-fatty acids; Ethyl esters; Solvent-free media; ACID-CATALYZED CONDENSATION; ENZYMATIC PRODUCTION; OLEIC-ACID; OIL; BIODIESEL; ESTOLIDES;
D O I：
10.1007/s00253-013-5059-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Novozym 435, lipase B from Candida antarctica, was used in this study for the production of ethyl esters. For the first time, trans-hydroxy-fatty acid ethyl esters were synthesized in vitro in solvent-free media. We studied the effects of the substrate-ethanol molar ratio and enzyme synthetic stability of the biocatalyst. To determine the structure of the formed compounds, Fourier transformed infrared spectroscopy, nuclear magnetic resonance, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry were used, three less time-consuming structural techniques. trans-Hydroxy-fatty acid ethyl esters were synthesized with a reaction yield of 90 % or higher with optimal reaction conditions.
引用
收藏
页码：8041 / 8048
页数：8
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