Development, characterisation and cross-species amplification of 16 novel microsatellite markers for the endangered Black-throated Finch (Poephila cincta) in Australia

被引:1
|
作者
Tang, Lei [1 ,2 ,3 ]
Smith-Keune, Carolyn [3 ]
Gardner, Michael G. [4 ,5 ,6 ]
Hardesty, Britta Denise [7 ]
机构
[1] James Cook Univ, Sch Earth & Environm Sci, Townsville, Qld 4811, Australia
[2] CSIRO Ecosyst Sci, Townsville, Qld 4811, Australia
[3] James Cook Univ, Sch Marine & Trop Biol, Townsville, Qld 4811, Australia
[4] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia
[5] Univ Adelaide, Australian Ctr Evolutionary Biol & Biodivers, Adelaide, SA 5000, Australia
[6] S Australian Museum, Evolutionary Biol Unit, Adelaide, SA 5000, Australia
[7] CSIRO Marine & Atmospher Res, Hobart, Tas 7000, Australia
关键词
Black-throated Finch; Poephila cincta; Microsatellite; Conservation genetics; Next generation sequencing; PROGRAM;
D O I
10.1007/s12686-013-0028-0
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
The Black-throated Finch (Southern) (Poephila cincta cincta) is threatened by the substantial landscape changes in northern Australia. We developed 16 polymorphic microsatellite markers using 454-shotgun whole-genome sequencing technology. We identified an average of 4.7 alleles per locus based on 63 wild caught individuals from Townsville, Queensland. Thirteen and 9 markers were also successfully cross-amplified in two confamilial species, the Double-barred Finch (Taeniopygia bichenovii) and the chestnut-breasted Mannikin (Lonchura castaneothorax) with 11 and 5 were polymorphic, respectively. These markers will help understand the population genetic structure of the endangered Black-throated Finch and determine genetic consequences of landscape changes for the species.
引用
收藏
页码:143 / 146
页数:4
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