SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145

被引：15
|
作者：
Ou, Xijun [1 ]
Zhang, Bo [1 ]
Zhang, Lin [1 ]
Dong, Kai [1 ]
Liu, Chun [1 ]
Zhao, Guoping [1 ,2 ,3 ]
Ding, Xiaoming [1 ]
机构：
[1] Fudan Univ, Sch Life Sci, Dept Microbiol & Microbial Engn, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
[2] Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 201203, Peoples R China
[3] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Mol Microbiol Lab, Shanghai 200032, Peoples R China
来源：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2008年 / 40卷 / 10期
基金：
中国国家自然科学基金;
关键词：
Streptomyces coelicolor; sporulation; antibiotic production; sarA;
D O I：
10.1111/j.1745-7270.2008.00466.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The filamentous bacteria Streptomyces exhibit a complex life cycle involving morphological differentiation and secondary metabolism. A putative membrane protein gene sarA (sco4069), sporulation and antibiotic production related gene A, was partially characterized in Streptomyces coelicolor M145. The gene product had no characterized functional domains and was highly conserved in Streptomyces. Compared with the wild-type M145, the sarA mutant accelerated sporulation and dramatically decreased the production of actinorhodin and undecylprodigiosin. Reverse transcription-polymerase chain reaction analysis showed that SarA influenced antibiotic production by controlling the abundance of actII-orf4 and redZ messenger RNA.
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页码：877 / 882
页数：6
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