Novel Strong Cation-exchange Monolithic Column Using Poly(vinyl ester resin) as Stationary Phase Materials for Lysozyme Separation by HPLC

A novel cation-exchange polymeric short column based on poly(vinyl ester resin) monolith was applied to separation in our present work. The poly(vinyl ester resin) was used as the monomer as well as the crosslinking agent in the polymerization which was induced by the initiator 2-methylpropropionitrile (AIBN). 1-Hexadecanol Was used its the porogen and methanol was used to make the 1-hexadecanol resolve more easily. Morphology of the monolithic materials was Studied by scanning electron microscopy. The capabilities of separation and loading on this column in conjunction with HPLC were investigated, respectively. The effect of pH and concentration of mobile phase (buffer) on the elution of Lys was investigated. Moreover, Lys was separated from chicken egg white with high resolution in it short time in this experiment.