Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosaminephosphate mutase from Candida albicans

被引：3
|
作者：
Nishitani, Y
Maruyama, D
Nonaka, T
Kita, A
Fukami, TA
Mio, T
Yamada-Okabe, H
Yamada-Okabe, T
Miki, K [1 ]
机构：
[1] Kyoto Univ, Grad Sch Sci, Dept Chem, Sakyo Ku, Kyoto 6068502, Japan
[2] Chugai Pharmaceut Co Ltd, Kamakura Res Lab, Kanagawa 2478530, Japan
[3] Yokohama City Univ, Sch Med, Dept Hyg, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[4] Harima Inst, RIKEN SPring 8 Ctr, Mikazuki, Hyogo 6785148, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2006年 / 62卷
关键词：
D O I：
10.1107/S1744309106010177
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
N-acetylglucosamine-phosphate mutase (AGM1) is an essential enzyme in the synthesis of UDP-N-acetylglucosamine ( UDP-GlcNAc) in eukaryotes and belongs to the alpha-D-phosphohexomutase superfamily. AGM1 from Candida albicans (CaAGM1) was purified and crystallized by the sitting-drop vapour-diffusion method. The crystals obtained belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 60.2, b = 130.2, c = 78.0 angstrom, beta = 106.7 degrees. The crystals diffract X-rays to beyond 1.8 angstrom resolution using synchrotron radiation.
引用
收藏
页码：419 / 421
页数：3
相关论文
共 50 条
  • [31] Purification, crystallization and preliminary X-ray studies of ClpX from Helicobacter pylori
    Kim, DY
    Wu, CA
    Kim, DR
    Ha, SC
    Han, YH
    Kim, KK
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2003, 59 : 1642 - 1644
  • [32] Expression, purification and preliminary X-ray diffraction studies of RebC
    van Staalduinen, Laura M.
    Bhattacharya, Anupam
    Groom, Katherine
    Zechel, David L.
    Jia, Zongchao
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2007, 63 : 980 - 982
  • [33] Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis
    Wang, Shanshan
    Nie, Yao
    Yan, Xu
    Ko, Tzu-Ping
    Huang, Chun-Hsiang
    Chan, Hsiu-Chien
    Guo, Rey-Ting
    Xiao, Rong
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2014, 70 : 800 - 802
  • [34] Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis
    Chen, Li-Chun
    Huang, Sheng-Cih
    Chuankhayan, Phimonphan
    Chen, Chung-Der
    Huang, Yen-Chieh
    Jeyakanthan, Jeyaraman
    Pang, Hsiao-Fang
    Men, Lee-Chung
    Chen, Yu-Ching
    Wang, Yu-Kuo
    Liu, Ming-Yih
    Wu, Tung-Kung
    Chen, Chun-Jung
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2009, 65 : 419 - 421
  • [35] Purification, crystallization and preliminary X-ray diffraction analysis of thermostable nitrile hydratase
    Tsekoa, TL
    Sayed, MF
    Cameron, RA
    Sewell, BT
    Cowan, DA
    SOUTH AFRICAN JOURNAL OF SCIENCE, 2004, 100 (9-10) : 488 - 490
  • [36] Purification, crystallization and preliminary X-ray diffraction analysis of human phosphoserine phosphatase
    Peeraer, Y
    Rabijns, A
    Verboven, C
    Collet, JF
    Van Schaftingen, E
    De Ranter, C
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 : 133 - 134
  • [37] The purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Clostridium botulinum
    Dobson, Renwick C. J.
    Atkinson, Sarah C.
    Gorman, Michael A.
    Newman, Janet M.
    Parker, Michael W.
    Perugini, Matthew A.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 206 - 208
  • [38] Expression, purification, crystallization and preliminary X-ray diffraction results from Campylobacter jejuni ferritin
    Clerté, S
    Dautant, A
    d'Estaintot, BL
    Gallois, B
    Mizunoe, Y
    Wai, SN
    Precigoux, G
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 : 299 - 301
  • [39] Purification, crystallization and preliminary X-ray diffraction data from selenomethionine glycinamide ribonucleotide synthetase
    Weaver, TM
    Wang, WR
    Ealick, SE
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1999, 55 : 518 - 521
  • [40] Purification, crystallization and preliminary X-ray diffraction experiment of nattokinase from Bacillus subtilis natto
    Yanagisawa, Yasuhide
    Chatake, Toshiyuki
    Chiba-Kamoshida, Kaori
    Naito, Sawa
    Ohsugi, Tadanori
    Sumi, Hiroyuki
    Yasuda, Ichiro
    Morimoto, Yukio
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 1670 - 1673
12345