Effect of tamoxifen on extracellular signal-regulated kinases in the urethra of castrated female rats

被引：1
|
作者：
dos Santos, Alesse R. [1 ]
Lopes-Costa, Pedro V. [1 ]
Gontijo, Jose A. [2 ]
da Silva, Benedito B. [1 ]
机构：
[1] Univ Fed Piaui, Dept Gynecol, Teresina, Piaui, Brazil
[2] Univ Estadual Campinas, Campinas, SP, Brazil
来源：
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY | 2012年 / 164卷 / 01期
关键词：
ERKs; Protein kinases; Rats; Tamoxifen; Urethra; ACTIVATED PROTEIN-KINASE; ESTROGEN-RECEPTOR; BREAST-CANCER; CELLS; PROLIFERATION; PHOSPHORYLATION; EXPRESSION; PATHWAYS; CASCADE; ERK;
D O I：
10.1016/j.ejogrb.2012.05.036
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: The aim was to evaluate the effects of tamoxifen in activating extracellular signal-regulated kinases (ERKs) 1 and 2 in the urethras of castrated female rats. Study design: Twelve castrated adult female rats were divided into a control group (n = 6) in which the animals received vehicle, and the experimental group (n = 6) in which the rats received tamoxifen 250 mu g/day by gavage for 28 days. Then, the animals were sacrificed and their urethras removed. Proteins were extracted, quantified and processed by Western blot analysis with specific phospho-ERK1 and 2 antibodies. Data were analyzed using Student's t-test (p < 0.05). Results: A significant increase occurred in phospho-ERK1 levels in the experimental group compared to the control group (p < 0.01), while no difference was found in phospho-ERK2 levels between the groups (p = 0.313). Conclusion: The present results indicate that, at the doses and during the time of treatment used, tamoxifen significantly increased phospho-ERK1 levels in the urethras of castrated female rats. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：102 / 104
页数：3
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