AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana

被引：60
|
作者：
Hirayama, T
Mitsukawa, N
Shibata, D
Shinozaki, K
机构：
[1] INST PHYS & CHEM RES,PLANT MOL BIOL LAB,TSUKUBA,IBARAKI 305,JAPAN
[2] MITSUI PLANT BIOTECHNOL RES INST,RITE 1,TSUKUBA LAB,TCI,D21,TSUKUBA,IBARAKI 305,JAPAN
来源：
PLANT MOLECULAR BIOLOGY | 1997年 / 34卷 / 01期
基金：
日本科学技术振兴机构;
关键词：
phosphoinositide-specific phospholipase C; chromosomal mapping; Arabidopsis thaliana;
D O I：
10.1023/A:1005885230896
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) from the higher plant Arabidopsis thaliana was cloned and characterized. The gene corresponding to this cDNA is designated AtPLC2. The overall structure of the predicted AtPLC2 protein is similar to those of plant PI-PLCs and mammalian delta-type PI-PLCs. Northern blot analysis revealed that AtPLC2 is expressed constitutively whereas AtPLC1S, another gene for PI-PLC of Arabidopsis, is induced by environmental stresses such as dehydration and salinity, indicating that the function of AtPLC2 is distinct from that of AtPLC1S. The AtPLC2 mRNA was detected in vegetative and floral tissues. We determined the positions of these two PI-PLCs genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.
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页码：175 / 180
页数：6
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