Protective effects of N-acetylcysteine in isoproterenol-induced myocardium injury in rats

被引:17
|
作者
Liu, Bin [2 ]
Li, Weimin [2 ]
Li, Yue [2 ]
Wang, Zhonghua [3 ]
Li, Hongli [4 ]
Liu, Ping [5 ]
Fu, Jun [1 ]
机构
[1] Harbin Med Coll, Clin Coll 1, Int Med Care Dept, Harbin 150001, Peoples R China
[2] Harbin Med Coll, Clin Coll 1, Cardiovasc Dept, Harbin 150001, Peoples R China
[3] Yangzhou Hongquan Hosp, Dept Cardiol, Yangzhou 225200, Peoples R China
[4] Shanghai Jiao Tong Univ, Dept Cardiol, Affiliated Peoples Hosp 1, Shanghai 200080, Peoples R China
[5] Harbin Med Coll, Dept Immunol, Harbin 150081, Peoples R China
关键词
Isoproterenol; N-acetylcysteine; Myocardium injury; Inflammatory factors; DNA-DAMAGE; CYSTEINE; INFARCTION; EXPRESSION; APOPTOSIS; CYTOKINE; CADMIUM; ALPHA; CELLS; MICE;
D O I
10.1007/s11033-008-9241-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Isoproterenol (ISO) has been found to cause severe injury in the myocardium. The aim of this study was to investigate the protective effects of N-acetylcysteine (NAC) on ISO-induced myocardial injury in rats and its underlying mechanisms. Fouty male Wistar rats were randomly divided into four groups: control, ISO, NAC, and ISO + NAC group. Myocardial histopathological observation were performed; The activities of creatine kinase isoenzyme-MB (CK-MB) and lactate dehydrogenase (LDH) were examined; Myocardium TNF-alpha and IL-1 beta gene expressions were examined by RT-PCR analysis; Myocardial expressions of TNF-alpha and IL-1 beta proteins were observed by immunohistochemical assay and western blotting analysis. The myocardial injury induced by ISO was significantly reduced by the treatment of NAC as judged by the reduction of myocardial necrosis. Compared with ISO group, rats pre-injected with NAC showed a significant decrease in the activities of cardiac marker enzymes such as CK-MB and LDH in serum. NAC inhibits the pro-inflammatory factors expressions (TNF-alpha and IL-1 beta) stimulated by ISO. In conclusion, NAC exerts significant cardio-protective effects against ISO-induced myocardial injury in rats, likely regulating pro-inflammatory factors expressions.
引用
收藏
页码:761 / 765
页数:5
相关论文
共 50 条
  • [1] Protective effects of N-acetylcysteine in isoproterenol-induced myocardium injury in rats
    Bin Liu
    Weimin Li
    Yue Li
    Zhonghua Wang
    Hongli Li
    Ping Liu
    Jun Fu
    [J]. Molecular Biology Reports, 2009, 36
  • [2] Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats
    Kahraman, Hasan
    Kurutas, Ergul
    Tokur, Mahmut
    Bozkurt, Selim
    Ciralik, Harun
    Kabakci, Betul
    Koksal, Nurhan
    Balkan, Vedat
    [J]. BALKAN MEDICAL JOURNAL, 2013, 30 (01) : 99 - 104
  • [3] Ghrelin protects myocardium from isoproterenol-induced injury in rats
    Chang, L
    Zhao, J
    Li, GZ
    Geng, B
    Pan, CS
    Qi, YF
    Tang, CS
    [J]. ACTA PHARMACOLOGICA SINICA, 2004, 25 (09) : 1131 - 1137
  • [4] Ghrelin protects myocardium from isoproterenol-induced injury in rats
    Lin CHANG
    Key Lab for Cardiovascular Biology of Education Ministry
    Department of Physiology
    [J]. Acta Pharmacologica Sinica, 2004, (09) : 29 - 35
  • [5] The Protective Effects of Compound 21 and Valsartan in Isoproterenol-Induced Myocardial Injury in Rats
    Ulutas, Zeynep
    Ermis, Necip
    Ozhan, Onural
    Parlakpinar, Hakan
    Vardi, Nigar
    Ates, Burhan
    Colak, Cemil
    [J]. CARDIOVASCULAR TOXICOLOGY, 2021, 21 (01) : 17 - 28
  • [6] The Protective Effects of Compound 21 and Valsartan in Isoproterenol-Induced Myocardial Injury in Rats
    Zeynep Ulutas
    Necip Ermis
    Onural Ozhan
    Hakan Parlakpinar
    Nigar Vardi
    Burhan Ates
    Cemil Colak
    [J]. Cardiovascular Toxicology, 2021, 21 : 17 - 28
  • [7] Protective effect of N-acetylcysteine in isoniazid induced hepatic injury in growing rats
    House No. 137/15-A, Chandigarh 160 015, India
    [J]. Indian Journal of Experimental Biology, 2001, 39 (05) : 436 - 440
  • [8] Protective Effects of 2-Methoxyestradiol on Acute Isoproterenol-Induced Cardiac Injury in Rats
    Hareeri, Rawan H.
    Alam, Abdulrahman M.
    Bagher, Amina M.
    Alamoudi, Abdulmohsin J.
    Aldurdunji, Mohammed M.
    Shaik, Rasheed A.
    Eid, Basma G.
    Ashour, Osama M.
    [J]. SAUDI PHARMACEUTICAL JOURNAL, 2023, 31 (10)
  • [9] Molecular and biochemical evidence on the protective effects of quercetin in isoproterenol-induced acutemyocardial injury in rats
    Kumar, Mukesh
    Kasala, Eshvendar Reddy
    Bodduluru, Lakshmi Narendra
    Kumar, Vikas
    Lahkar, Mangala
    [J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2017, 31 (01)
  • [10] Protective Effects of Cardamom in Isoproterenol-Induced Myocardial Infarction in Rats
    Goyal, Sameer N.
    Sharma, Charu
    Mahajan, Umesh B.
    Patil, Chandragouda R.
    Agrawal, Yogeeta O.
    Kumari, Santosh
    Arya, Dharamvir Singh
    Ojha, Shreesh
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (11) : 27457 - 27469