Synthesis of oligodeoxynucleotides containing 4′-C-aminoalkylthymidines and their thermal stability and nuclease-resistance properties

被引：1

作者：

Ueno, Y ^{[1
]}

Kanazaki, M ^{[1
]}

Shuto, S ^{[1
]}

Matsuda, A ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Pharmaceut Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan

来源：

NUCLEOSIDES & NUCLEOTIDES | 1999年 / 18卷 / 6-7期

关键词：

D O I：

10.1080/07328319908044731

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To find the nuclease-resistant oligodeoxynucleotides (ODNs) with natural phosphodiester linkages, we designed and synthesized ODNs containing 4'-C-aminoalkylthymidines (1-4). We found that the ODNs containing 1, 2, 3 or 4 were more resistant to nucleolytic hydrolysis by both snake venom phosphodiesterase (a 3'-exonuclease) and DNase I (an endonuclease) than unmodified ODNs.

引用

页码：1401 / 1402

页数：2

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