Reconstruction of complementary images in second harmonic generation microscopy

被引：21
|
作者：
Gao, Liang [1 ]
Jin, Lei
Xue, Ping
Xu, Jun
Wang, Yi
Ma, Hui
Chen, Dieyan
机构：
[1] Tsinghua Univ, Dept Phys, Lab Atom Mol & Nanosci, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Dept Chem Engn, Beijing 100084, Peoples R China
来源：
OPTICS EXPRESS | 2006年 / 14卷 / 11期
关键词：
D O I：
10.1364/OE.14.004727
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Second harmonic generation microscopy(SHGM) has become widely used to image biological samples. Due to the complexity of biological samples, more and more effort has been put on polarization imaging in SHGM technology to uncover their structures. In this work, we put forward a novel stitching method based on careful mathematical calculation, and accomplish it by rotating laser polarization. We first show its validity in imaging a perfectly synthesized bio-origin polymer poly (3-hyroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Then, we test its power by getting a true image of fibrillar collagen structure of rat-tail tendon. (c) 2006 Optical Society of America.
引用
收藏
页码：4727 / 4735
页数：9
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