Optical Control of a Biological Reaction-Diffusion System

被引：21

作者：

Glock, Philipp ^{[1
]}

Broichhagen, Johannes ^{[2
,3
]}

Kretschmer, Simon ^{[1
]}

Blumhardt, Philipp ^{[1
]}

Muecksch, Jonas ^{[1
]}

Trauner, Dirk ^{[2
,4
]}

Schwille, Petra ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Cellular & Mol Biophys, Klopferspitz 18, D-82152 Martinsried, Germany

[2] Ludwig Maximilians Univ Munchen, Dept Chem, Butenandtstr 5-13, D-81377 Munich, Germany

[3] Max Planck Inst Med Res, Dept Chem Biol, Jahnstr 29, D-69120 Heidelberg, Germany

[4] NYU, Dept Chem, Silver Ctr Arts & Sci, 100 Washington Sq East, New York, NY 10003 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2018年 / 57卷 / 09期

关键词：

chemical oscillators; optical control; photoswitches; pattern formation; synthetic biology; CELL-DIVISION; ESCHERICHIA-COLI; PATTERN-FORMATION; CHEMICAL-SYSTEM; MINE; PROTEINS; FEEDBACK; PEPTIDE; WAVES;

D O I：

10.1002/anie.201712002

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Patterns formed by reaction and diffusion are the foundation for many phenomena in biology. However, the experimental study of reaction-diffusion (R-D) systems has so far been dominated by chemical oscillators, for which many tools are available. In this work, we developed a photoswitch for the Min system of Escherichia coli, a versatile biological in vitro R-D system consisting of the antagonistic proteins MinD and MinE. A MinE-derived peptide of 19 amino acids was covalently modified with a photoisomerizable crosslinker based on azobenzene to externally control peptide-mediated depletion of MinD from the membrane. In addition to providing an on-off switch for pattern formation, we achieve frequency-locked resonance with a precise 2D spatial memory, thus allowing new insights into Min protein action on the membrane. Taken together, we provide a tool to study phenomena in pattern formation using biological agents.

引用

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页码：2362 / 2366

页数：5

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