Isolation and characterization of bacteria from the midgut of the Asian honey bee (Apis cerana indica)

被引：15
|
作者：
Disayathanoowat, Terd [1 ]
Yoshiyama, Mikio [2 ]
Kimura, Kiyoshi [2 ]
Chantawannakul, Panuwan [1 ,3 ]
机构：
[1] Chiang Mai Univ, Fac Sci, Dept Biol, Bee Protect Ctr, Chiang Mai 50000, Thailand
[2] Natl Agr & Food Res Org NARO, Honey Bee Res Unit, Anim Breeding & Reprod Res Div, NARO Inst Livestock & Grassland Sci, Tsukuba, Ibaraki, Japan
[3] Chiang Mai Univ, Fac Sci, Mat Sci Res Ctr, Chiang Mai 50000, Thailand
来源：
JOURNAL OF APICULTURAL RESEARCH | 2012年 / 51卷 / 04期
关键词：
Apis cerana; Asian cavity nesting honey bee; culture dependent method; microflora; aerobic bacteria; PAENIBACILLUS-LARVAE; AMERICAN FOULBROOD; CAUSAL AGENT; GUT BACTERIA; MELLIFERA; IDENTIFICATION; INHIBITION; KLEBSIELLA; DIVERSITY;
D O I：
10.3896/IBRA.1.51.4.04
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
Aerobic bacteria were isolated from the midgut of Apis cerana indica on five different media. The highest count (5.4 x 10(7) colony forming units (CFU/g)) was obtained on Eosin methylene blue (EMB) medium. Fifty-eight isolates were purified and identified using biochemical tests, and 16S rRNA gene sequences were analysed. Two groups of Gamma-proteobacteria and Firmicutes were found. The most abundant species were Klebsiella pneumoniae, Enterobacter cloacae and K. oxytoca. To determine their role in nutrition, enzymes produced from the bacterial isolates were assayed. Protease and lipase activities were detected in some bacterial isolates. The assimilation of fourteen carbon substrates was studied. D-mannose and raffinose showed the highest utilization. Paenibacillus larvae, the cause of American foulbrood disease, was inhibited by some members of Klebsiella.
引用
收藏
页码：312 / 319
页数：8
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