A new detection method for a newly revealed mechanism of pyrethroid resistance development in Varroa destructor

被引：14
|
作者：
Strachecka, Aneta [1 ]
Borsuk, Grzegorz [1 ]
Olszewski, Krzysztof [1 ]
Paleolog, Jerzy [1 ]
机构：
[1] Univ Life Sci Lublin, Fac Biol & Anim Breeding, Dept Biol Basis Anim Prod, PL-20950 Lublin, Poland
来源：
PARASITOLOGY RESEARCH | 2015年 / 114卷 / 11期
关键词：
Varroa destructor; PCR-SSCP; Acaricides; Mitochondrial cytochrome oxidase I (COI); Drug resistance; SODIUM-CHANNEL GENE; DNA METHYLATION; JACOBSONI OUD; MOLECULAR CHARACTERIZATION; FLUVALINATE-RESISTANCE; BODY-SURFACE; LIFE-SPAN; PCR-SSCP; MITE; KDR;
D O I：
10.1007/s00436-015-4627-4
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The Varroa destructor mite has recently displayed an ever increasing resistance to new drugs, contributing to CCD proliferation. This work was aimed at determining new viable methods for identifying the pyrethroid resistance of V. destructor and DNA methylation in resistant and sensitive mites. DNA was extracted from Varroa mites. Nucleotide changes in the DNA of pyrethroid-resistant, pyrethroid-sensitive, and control mites were identified with polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) in the case of five mitochondrial gene fragments. More bands were observed in the drug-resistant mites than in the other two groups. Sequencing confirmed these observations. Decreased global DNA methylation levels were observed in the pyrethroid-resistant mites. There exists a previously undescribed mechanism of pyrethroid resistance development in Varroa mites. The PCR-SSCP methods can be considered and further developed as useful tools for detecting V. destructor resistance.
引用
收藏
页码：3999 / 4004
页数：6
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