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An optosensor for tryptophan with C18 silica gel as a substrate
被引:0
|作者:
Gong, ZL
Zhang, ZJ
机构:
[1] SHAANXI NORMAL UNIV,DEPT CHEM,XIAN 710062,PEOPLES R CHINA
[2] NANJING UNIV,DEPT CHEM,NANJING 210008,PEOPLES R CHINA
来源:
基金:
中国国家自然科学基金;
关键词:
D O I:
10.1007/s002160050312
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
A flow-through optosensor for tryptophan with C18 silica gel as a substrate is proposed. It has been developed in conjunction with a flow-injection analysis system and is based on the retention of tryptophan on the C18 column and the enhancement of its fluorescence. The detection limit is 25 ng mL(-1) with a relative standard deviation of 2.9% for 7 determinations of 10 mu g mL(-1) of tryptophan. Most of the common species do not interfere. The method has been successfully applied to a pharmaceutical preparation.
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页码:1097 / 1100
页数:4
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