Sulfur based self-assembled monolayers (SAM's) on piezoelectric crystals for immunosensor development

被引：55
|
作者：
Vaughan, RD [1 ]
O'Sullivan, CK [1 ]
Guilbault, GG [1 ]
机构：
[1] Natl Univ Ireland Univ Coll Cork, Dept Chem, Lab Sensor Dev, Cork, Ireland
来源：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 364卷 / 1-2期
关键词：
D O I：
10.1007/s002160051300
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Self-assembled monolayers (SAM's) of different thiols and sulfides with various structures were tested. They were investigated as a method to covalently bind antibodies to the surface of piezoelectric crystals. A capture assay using a Mouse IgG test system was performed. All tests were performed in solution. The reduction in frequency observed as binding occurred was monitored in real time. The SAM's were activated with 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (NHS) prior to antibody immobilisation. Ethanolamine was used to block any available activated sizes after antibody immobilisation. The potential of this technique for immunosensor development is demonstrated.
引用
收藏
页码：54 / 57
页数：4
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