Localization of metal ions in biomolecules by means of pulsed dipolar EPR spectroscopy

被引：7

作者：

Abdullin, Dinar ^{[1
]}

Schiemann, Olav ^{[1
]}

机构：

[1] Univ Bonn, Inst Phys & Theoret Chem, Wegelerstr 12, D-53115 Bonn, Germany

来源：

DALTON TRANSACTIONS | 2021年 / 50卷 / 03期

关键词：

ELECTRON DOUBLE-RESONANCE; NITROXIDE SPIN-LABELS; DISTANCE MEASUREMENTS; HAMMERHEAD RIBOZYME; ORIENTATION SELECTION; BAND INVERSION; HIGH-AFFINITY; BINDING-SITE; BROAD-BAND; PELDOR;

D O I：

10.1039/d0dt03596c

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Metal ions are important for the folding, structure, and function of biomolecules. Thus, knowing where their binding sites are located in proteins or oligonucleotides is a critical objective. X-ray crystallography and nuclear magnetic resonance are powerful methods in this respect, but both have their limitations. Here, a complementary method is highlighted in which paramagnetic metal ions are localized by means of trilateration using a combination of site-directed spin labeling and pulsed dipolar electron paramagnetic resonance spectroscopy. The working principle, the requirements, and the limitations of the method are critically discussed. Several applications of the method are outlined and compared with each other.

引用

页码：808 / 815

页数：8

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