The purB gene of Escherichia coli K-12 is located in an operon

被引：20
|
作者：
Green, SM [1 ]
Malik, T [1 ]
Giles, IG [1 ]
Drabble, WT [1 ]
机构：
[1] UNIV SOUTHAMPTON,DEPT BIOCHEM,SOUTHAMPTON SO16 7PX,HANTS,ENGLAND
来源：
MICROBIOLOGY-UK | 1996年 / 142卷
关键词：
Escherichia coli; purB gene; succinyl-AMP lyase; internal promoter; phoP gene;
D O I：
10.1099/13500872-142-11-3219
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The structural gene (purB) for succinyl-AMP (S-AMP) lyase and three additional ORFs are on the same DNA strand of the chromosome of Escherichia coli. Cassette mutagenesis and primer extension mapping demonstrated that purB is co-transcribed with an upstream gene (ORF23. or ycfC) encoding a 22.9 kDa membrane-associated protein of non-essential. but unknown, function unrelated to purine biosynthesis. The purB operon lies between phoP and an ORF expressing an essential function which may correspond to asuE (trmU). S-AMP lyase was purified to near homogeneity. The purified enzyme is a homotetramer of 50 kDa subunits, has a K-m for S-AMP of 3.7 mu M and a pH optimum of 7.4-7.6.
引用
收藏
页码：3219 / 3230
页数：12
相关论文
共 50 条
  • [11] A mutation in a new gene, bglJ, activates the bgl operon in Escherichia coli K-12
    Giel, M
    Desnoyer, M
    Lopilato, J
    GENETICS, 1996, 143 (02) : 627 - 635
  • [12] PHYSICAL CHARACTERIZATION OF THE ILVHI OPERON OF ESCHERICHIA-COLI K-12
    SQUIRES, CH
    DEFELICE, M
    WESSLER, SR
    CALVO, JM
    JOURNAL OF BACTERIOLOGY, 1981, 147 (03) : 797 - 804
  • [13] HIST IS PART OF A MULTIGENE OPERON IN ESCHERICHIA-COLI K-12
    MARVEL, CC
    ARPS, PJ
    RUBIN, BC
    KAMMEN, HO
    PENHOET, EE
    WINKLER, ME
    JOURNAL OF BACTERIOLOGY, 1985, 161 (01) : 60 - 71
  • [14] TRANSLATIONAL COUPLING IN THE THREONINE OPERON OF ESCHERICHIA-COLI K-12
    LITTLE, S
    HYDE, S
    CAMPBELL, CJ
    LILLEY, RJ
    ROBINSON, MK
    JOURNAL OF BACTERIOLOGY, 1989, 171 (06) : 3518 - 3522
  • [15] MOLECULAR CHARACTERIZATION OF THE TDC OPERON OF ESCHERICHIA-COLI K-12
    GOSS, TJ
    SCHWEIZER, HP
    DATTA, P
    JOURNAL OF BACTERIOLOGY, 1988, 170 (11) : 5352 - 5359
  • [16] SECONDARY PROMOTER OF GUANINE OPERON OF ESCHERICHIA-COLI K-12
    FUKUMAKI, Y
    SHIMADA, K
    TAKAGI, Y
    JAPANESE JOURNAL OF GENETICS, 1977, 52 (06): : 439 - 439
  • [17] MAPPING OF ASPARTASE GENE IN ESCHERICHIA COLI K-12
    MARCUS, M
    HALPERN, YS
    ISRAEL JOURNAL OF MEDICAL SCIENCES, 1969, 5 (03): : 413 - &
  • [18] THE NUCLEOTIDE-SEQUENCE OF RECG, THE DISTAL SPO OPERON GENE IN ESCHERICHIA-COLI K-12
    KALMAN, M
    MURPHY, H
    CASHEL, M
    GENE, 1992, 110 (01) : 95 - 99
  • [19] NUCLEOTIDE-SEQUENCE OF THE GUABA OPERON OF ESCHERICHIA-COLI K-12
    TIEDEMAN, AA
    SMITH, JM
    FEDERATION PROCEEDINGS, 1985, 44 (05) : 1419 - 1419
  • [20] GENETIC-ANALYSIS OF PST OPERON IN ESCHERICHIA-COLI K-12
    AMEMURA, M
    MAKINO, K
    SHINAGAWA, H
    NAKATA, A
    JAPANESE JOURNAL OF GENETICS, 1985, 60 (06): : 582 - 582
12345