Molecular cloning, characterization and expression pattern of a novel dihydroflavonol-4-reductase gene

被引：0
|
作者：
Zeng Jianmin [1 ]
Nian Fuzhao [2 ]
机构：
[1] Yunnan Acad Tobacco Agr Sci, Yuxi 653100, Peoples R China
[2] Yunnan Agr Univ, Coll Tobacco Sci, Kunming 650201, Peoples R China
来源：
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2015年 / 10卷 / 10期
关键词：
Tobacco; gene; DFR; expression pattern; FLAVONOIDS;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Anthocyanins have significant health benefits for animals and humans. Dihydroflavonol-4-reductase (DFR) has been characterized to a key enzyme in anthocyanin biosynthesis. The complete coding sequence of tobacco DFR gene was amplified by RTPCR. The complete coding sequence of tobacco DFR gene was 990bp which encodes a protein of 329 amino acids. Sequence analysis revealed that the DFR of tobacco shares high homology with the DFR of wine grape (58%), soybean (58%), populus trichocarpa (56%) and barrel medic (56%). Phylogenetic tree analysis revealed that the tobacco DFR gene has a closer genetic relationship with that of soybean. Expression profile was studied and the results indicated that tobacco DFR gene was highly expressed in leaf and flower. These results established the primary foundation of utilizing tobacco anthocyanins as drugs for animals and humans in the future.
引用
收藏
页码：94 / 98
页数：5
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