Genetic control of alcohol dehydrogenase, malate dehydrogenase, and phosphoglucomutase isozymes in lima bean (Phaseolus lunatus L)

被引：3
|
作者：
Bi, IZ [1 ]
Maquet, A [1 ]
Wathelet, B [1 ]
Baudoin, JP [1 ]
机构：
[1] FAC UNIV SCI AGRON GEMBLOUX,UER CHIM ORGAN & BIOL,B-5030 GEMBLOUX,BELGIUM
来源：
PLANT BREEDING | 1997年 / 116卷 / 02期
关键词：
Phaseolus lunatus; distorted segregation; inheritance; isozyme analysis; linkage;
D O I：
10.1111/j.1439-0523.1997.tb02175.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
A suitable electrophoretic separation method for alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), and phosphoglucomutase (PGM) from P. lunatus has been developed. Two loci (Adh-2 and Pgm-2) showed codominant inheritance and fitted Mendelian ratio. ADH isozyme banding patterns indicate a dimeric quaternary structure, while those of PGM were in agreement with a monomeric nature. The cytoplasmic location of two MDH isozymes (Mdh-1 and Mdh-2) selectively inactivated by homogenization in an ascorbic acid solution was demonstrated. However, distorted ratios were observed for Mdh-2 segregation. On the basis of MDH isozyme banding patterns observed in five progeny families, it is suggested that this enzyme system is a dimeric protein encoded by at least three codominant genes (Mdh-1, Mdh-2, and Mdh-3). Joint segregation tests between pairs of segregating loci (Adh-2, Mdh-2, and Pgm-2) indicated that each of them is inherited independently.
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页码：181 / 185
页数：5
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