CHARACTERIZATION OF WHEAT GENOTYPES USING RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS

被引:0
|
作者
Bibi, Khatiba [1 ]
Inamullah [1 ]
Ahmad, Habib [1 ]
Din, Sirajud [3 ]
Muhammad, Fida [2 ]
Iqbal, Muhammad Sajjad [1 ]
机构
[1] Hazara Univ, Dept Genet, Mansehra, Pakistan
[2] NW Frontier Prov Agr Univ, Dept Genet & Plant Breeding, Peshawar, Pakistan
[3] Univ Peshawar, Dept Bot, Peshawar, Pakistan
关键词
GENETIC DIVERSITY;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Genomic DNA from the genotypes Khyber-87, Saleem 2000, Suleman-96, Pie Sabak-2004, Pir Sabak-2005, NRL-0320, NRL-0517, RAS-II, Tatara, RAS-I, Balhoor, Fakhare Sarhad, Bakhtawar-94, Inqilab-91, Haider-2002, Noshera-96, and Auqab-2000 was amplified using RAID primers. Loci of 100-1400 bp sizes were amplified and on average 3.51 loci per genotype were amplified. Average genetic diversity using the twelve RAPD primers ranged from 30-90%. Phylogenetic relationship among the wheat genotypes was carried out using (dendrogram analysis. The seventeen genotypes were clustered in live groups. It was found that the genotypes Pirsabak 2004 and Ras 1 were most distantly related to Khyber-87. It is suggested that these two genotypes be crossed with Khyber 87 for creating maximum genetic variations.
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页码:1509 / 1512
页数:4
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