CHARACTERIZATION OF WHEAT GENOTYPES USING RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS

Genomic DNA from the genotypes Khyber-87, Saleem 2000, Suleman-96, Pie Sabak-2004, Pir Sabak-2005, NRL-0320, NRL-0517, RAS-II, Tatara, RAS-I, Balhoor, Fakhare Sarhad, Bakhtawar-94, Inqilab-91, Haider-2002, Noshera-96, and Auqab-2000 was amplified using RAID primers. Loci of 100-1400 bp sizes were amplified and on average 3.51 loci per genotype were amplified. Average genetic diversity using the twelve RAPD primers ranged from 30-90%. Phylogenetic relationship among the wheat genotypes was carried out using (dendrogram analysis. The seventeen genotypes were clustered in live groups. It was found that the genotypes Pirsabak 2004 and Ras 1 were most distantly related to Khyber-87. It is suggested that these two genotypes be crossed with Khyber 87 for creating maximum genetic variations.