Batch and continuous hydrolysis of lactose using β-galactosidase immobilized on gelatin-CMC

被引：9

作者：

Sungur, S ^{[1
]}

Yildirim, Ö

机构：

[1] Ankara Univ, Fac Sci, Dept Chem, TR-06100 Ankara, Turkey

[2] Ankara Univ, Fac Sci, Dept Biol, TR-06100 Ankara, Turkey

来源：

POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING | 1999年 / 38卷 / 04期

关键词：

D O I：

10.1080/03602559909351616

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

beta-Galactosidase enzyme (E,C. 3.2.1.23) was immobilized onto a gelatin-carboxymethylcellulose carrier system by using cross-linker chromium acetate, A relative activity increase of 33% was achieved by adding carboxymethylcellulose to gelatin, The effect of operation temperature and pH on relative activity and reusability of the immobilized enzyme was investigated, A fluidized-bed reactor with a downflow mechanism was used for continuous process hydrolysis, and a constant glucose production rate was obtained.

引用

页码：821 / 829

页数：9

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